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Most Viewed Protocols

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Differentiation of Mouse Embryonic Stem Cells Into Endothelial Cells

16323 views
Generation of Osteoblasts and Chondrocytes From Embryonic Stem Cells

16098 views
Isolation and Functional Characterization of Side Population Stem Cells

13410 views
Experimental Models to Analyze Differentiation Functions of Cultured Keratinocytes In Vitro and In Vivo

12490 views
The Role of Hex in Hemangioblast and Hematopoietic Development

2674 views
Differentiation and Characterization of Human MSCs

2634 views
Formation of Gut-Like Structures In Vitro From Mouse Embryonic Stem Cells

2581 views
Isolation, Characterization, and Culture of Epithelial Stem Cells

2402 views
Derivation and Characterization of Hepatocytes From Embryonic Stem Cells In Vitro

2391 views
In Vitro Fabrication of Engineered Human Skin

2348 views
FACS Enrichment of Human Keratinocyte Stem Cells

2326 views
Gene Delivery to Mesenchymal Stem Cells

2302 views
Derivation and Characterization of Gut-Like Structures From Embryonic Stem Cells

2287 views
Biological Scaffolds for Heart Valve Tissue Engineering

2219 views
Cardiomyocytes From Human and Mouse Embryonic Stem Cells

2103 views
Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures

2084 views
Labeling Stem Cells In Vitro for Identification of their Differentiated Phenotypes after Grafting into the CNS

2066 views
Artificial Skin

2066 views
Immune Properties of Mesenchymal Stem Cells

2049 views
Colony Forming Unit Assays for MSCs

2046 views
Spermatogonial Stem Cell Transplantation, Testicular Function, and Restoration of Male Fertility in Mice

2042 views
Small Blood Vessel Engineering

2041 views
Immunocytochemical Analysis of Neuronal Differentiation

2028 views
Analysis of the C. elegans Germline Stem Cell Region

2003 views
Study Origin of Germ Cells and Formation of New Primary Follicles in Adult Human and Rat Ovaries

2002 views
Transplantation of Chimeric Fetal Liver to Study Hematopoiesis

1979 views
Assays of MSCs with Microarrays

1944 views
Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold

1931 views
Analytical Methods for Cancer Stem Cells

1881 views
Clinical Grade Expansion of Human Bone Marrow Mesenchymal Stem Cells

1874 views
High-Resolution Light Microscopic Characterization of Spermatogonia

1834 views
Hematopoietic Stem Cell Transplant into Non-Myeloablated W/W v Mice to Detect Steady-State Engraftment Defects

1763 views
StemBase

1743 views
Immunodeficient Mouse Models to Study Human Stem Cell-Mediated Tissue Repair

1731 views
In Vitro Derivation and Expansion of Endothelial Cells From Embryonic Stem Cells

1034 views
Isolation and Culture of Bone Marrow-Derived Human Multipotent Stromal Cells (hMSCs)

932 views
Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells

835 views
In Vitro Assays for Cobblestone Area-Forming Cells, LTC-IC, and CFU-C

790 views
Isolation of Stem Cells from Human Umbilical Cord Blood

771 views
Derivation and Propagation of Embryonic Stem Cells in Serum- and Feeder-Free Culture

767 views
Isolation of Human Adipose-derived Stem Cells from Biopsies and Liposuction Specimens

762 views
Proliferation, Differentiation, and Inflammation in Normal and Hyperproliferative Skin Using Multiparameter Flow Cytometry

732 views
Digital Imaging of Stem Cells by Electron Microscopy

696 views
Isolation of Recombinant Phage-Displayed Antibodies Recognizing Skin Keratinocytes

694 views
Optimizing Stem Cell Grafting into the CNS

691 views
Two-Photon Fluorescence Imaging and Reactive Oxygen Species Detection Within the Epidermis

680 views
Retroviral Transduction of Murine Hematopoietic Stem Cells

677 views
Gene Targeting by Oligonucleotides in Keratinocytes

666 views
The Analysis of Mitochondria and Mitochondrial DNA in Human Embryonic Stem Cells

660 views
Retroviral Transduction of Hematopoietic Progenitors Derived From Human Embryonic Stem Cells

660 views
Derivation and Characterization of Hematopoietic Cells From Human Embryonic Stem Cells

659 views
Keratinocyte Culture in the Absence of Substrate Attachment

646 views
Generation of Multicellular Tumor Spheroids by the Hanging-Drop Method

637 views
Organ Culture of Developing Mouse Skin and Its Application for Molecular Mechanistic Studies of Morphogenesis

632 views
Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis

631 views
Immunohistological Techniques for Studying the Drosophila Male Germline Stem Cell

629 views
Fluorimetric DNA Assay of Cell Number

623 views
Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts

623 views
Flow Cytometry-Based Cell Cycle Measurement of Mouse Hematopoietic Stem and Progenitor Cells

617 views
Skeletal (“Mesenchymal”) Stem Cells for Tissue Engineering

614 views
Method for the Harvest and Assay of In Vitro Clonogenic Keratinocytes Stem Cells From Mice

612 views
A Method to Isolate and Purify Human Bone Marrow Stromal Stem Cells

610 views
Isolation of Mesenchymal Stem Cells from Murine Bone Marrow by Immunodepletion

608 views
Cartilage Tissue Engineering

606 views
A Controlled-Cooling Protocol for Cryopreservation of Human and Non-Human Primate Embryonic Stem Cells

605 views
Targeted Somatic Mutagenesis in the Mouse Epidermis

605 views
Epigenetic Control in Male Germ Cells

602 views
Freezing Harvested hMSCs and Recovery of hMSCs from Frozen Vials for Subsequent Expansion, Analysis, and Experimentation

600 views
Designing, Testing, and Validating a Microarray for Stem Cell Characterization

599 views
The Development of Germline Stem Cells in Drosophila

599 views
Isolation of Neural Stem and Precursor Cells from Rodent Tissue

599 views
Structural Polarity and Dynamics of Male Germline Stem Cells in an Insect (Milkweed Bug Oncopeltus fasciatus )

596 views
In Vitro Expansion of Tissue Cells by Conditional Proliferation

596 views
Culture and Characterization of Human Bone Marrow Mesenchymal Stem Cells

591 views
Gene and Stem Cell Therapy of the Hair Follicle

591 views
Gene Expression Analysis at the Single Cell Level Using the Human Bone Marrow Stromal Cell as a Model: Sample Preparation Methods

591 views
Generation of a Monoclonal Antibody Library Against Human Embryonic Stem Cells

589 views
Application of Genetically Modified Feeder Cells for Culture of Keratinocytes

585 views
In Vivo Transplantation of Engineered Human Skin

584 views
Epidermis-Targeted Gene Transfer Using In Vivo Electroporation

583 views
Testicular Germ Cell Tumors in Mice

582 views
Human Articular Chondrocytes Culture

582 views
Myocardial Restoration and Tissue Engineering of Heart Structures

580 views
Identification and Characterization of Spermatogonial Subtypes and Their Expansion in Whole Mounts and Tissue Sections from Primate Testes

578 views
Biomaterials/Scaffolds

575 views
In Vitro Assays for Neural Stem Cell Differentiation: Induction of Dopaminergic Phenotype

569 views
Deriving Mouse Spermatogonial Stem Cell Lines

567 views
In Vitro Heart Valve Tissue Engineering

563 views
A Novel Experimental Platform for Investigating Cancer Growth and Anti-Cancer Therapy in a Human Tissue Microenvironment Derived From Human Embryonic Stem Cells

562 views
In Vitro Migration Assays of Neural Stem Cells

558 views
GDNF Maintains Mouse Spermatogonial Stem Cells In Vivo and In Vitro

555 views
Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes

554 views
Bioluminescence Imaging of Hematopoietic Stem Cell Repopulation in Murine Models

554 views
Analysis of Apoptosis in Hematopoietic Stem Cells by Flow Cytometry

553 views
Maintenance of Human Embryonic Stem Cells in Animal Serum- and Feeder Layer-Free Culture Conditions

551 views
Keratin 19 as a Stem Cell Marker In Vivo and In Vitro

547 views
Isolating Highly Pure Rat Spermatogonial Stem Cells in Culture

546 views
Mesenchymal Stem Cells from Adult Bone Marrow

544 views
Human Embryonic Stem Cells for Tissue Engineering

544 views
Transplantation of Human Embryonic Stem Cells to the Chick Embryo

538 views
Synthetic Hydrogel Matrices for Guided Bladder Tissue Regeneration

536 views
In Vitro Vascularization of Human Connective Microtissues

531 views
Genetic Manipulation of Human Embryonic Stem Cells by Transfection

529 views
In Vivo Labeling and Analysis of Epidermal Stem Cells

524 views
Telomere Neurobiology

521 views
RNAi Knockdown of Transcription Factor Pu.1 in the Differentiation of Mouse Embryonic Stem Cells

519 views
Measuring Apoptosis in Neural Stem Cells

517 views
Derivation and Characterization of Thyrocyte-Like Cells From Embryonic Stem Cells In Vitro

516 views
Mobilization of Hematopoietic Stem and Progenitor Cells in Mice

516 views
In Vitro Differentiation of Embryonic Stem Cells Into the Pancreatic Lineage

511 views
Integrins and Vascular Development in Differentiated Embryonic Stem Cells In Vitro

508 views
Stem Cell Engineering Using Transducible Cre Recombinase

502 views
Confrontation Cultures of Embryonic Stem Cells With Multicellular Tumor Spheroids to Study Tumor-Induced Angiogenesis

501 views
Electroporation Strategies for Genetic Manipulation and Cell Labeling

497 views
Human Embryonic Stem Cells

493 views
Practical Aspects of Cardiac Tissue Engineering With Electrical Stimulation

491 views
Artificial Pancreas to Treat Type 1 Diabetes Mellitus

490 views
Cell Surface Markers in Human Embryonic Stem Cells

489 views
In Vitro Human T Cell Development Directed by Notch–Ligand Interactions

488 views
Derivation and Characterization of Lentoid Bodies and Retinal Pigment Epithelial Cells From Monkey Embryonic Stem Cells In Vitro

488 views
Microfluidic Devices for High-Throughput Gene Expression Profiling of Single hESC-Derived Neural Stem Cells

487 views
Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo

487 views
Adipose-Derived Stem Cells

484 views
Isolation of Quiescent Murine Hematopoietic Stem Cells by Homing Properties

481 views
Markers of Adult Neural Stem Cells

480 views
Derivation and Maintenance of Human Embryonic Stem Cells

477 views
Culture of Human Embryonic Stem Cells on Human and Mouse Feeder Cells

476 views
Hematopoietic Development of Human Embryonic Stem Cells in Culture

474 views
Lentiviral Gene Transduction of Mouse and Human Stem Cells

473 views
Generation of Chondrocytes From Embryonic Stem Cells

473 views
TGF-β Signaling in Embryonic Stem Cell-Derived Endothelial Cells

473 views
Isolation of Oligodendroglial Cells from Cultured Neural Stem/Progenitors

472 views
Efficient Generation of Dopamine Neurons from Human Embryonic Stem Cells

471 views
Derivation of Human Feeders for Prolonged Support of Human Embryonic Stem Cells

470 views
Neural Differentiation of Human Embryonic Stem Cells

467 views
Adenoviral Transduction of Mesenchymal Stem Cells

466 views
In Vitro Differentiation of Neural Precursors From Human Embryonic Stem Cells

466 views
Generating Neurons from Stem Cells

465 views
Differentiation of Rhesus Monkey Embryonic Stem Cells in Three-Dimensional Collagen Matrix

464 views
Differentiation of Embryonic Stem Cells to Retinal Cells In Vitro

463 views
Routine Culture and Differentiation of Human Embryonic Stem Cells

463 views
Chromosomal Mosaicism in Neural Stem Cells

462 views
Routes of Stem Cell Administration in the Adult Rodent

462 views
Derivation and Characterization of Neuronal Precursors and Dopaminergic Neurons From Human Embryonic Stem Cells In Vitro

460 views
Derivation and Characterization of Chondrocytes From Embryonic Stem Cells In Vitro

458 views
Retroviral Integration Site Analysis in Hematopoietic Stem Cells

449 views
Blastocyst Culture for Deriving Human Embryonic Stem Cells

447 views
Generation and Characterization of Oligodendrocytes From Lineage-Selectable Embryonic Stem Cells In Vitro

447 views
Propagation of Human Embryonic Stem Cells on Human Feeder Cells

446 views
Hematopoietic Stem Cell Transplant in Mice by Intra-Femoral Injection

445 views
In Vitro Hematopoietic Differentiation of Murine Embryonic Stem Cells

444 views
Neural Stem Sphere Method

442 views
Insulin-Producing Cells from Embryonic Stem Cells Experimental Considerations

440 views
Study of Gap Junctions in Human Embryonic Stem Cells

439 views
Novel and Immortalization-Based Protocols for the Generation of Neural CNS Stem Cell Lines for Gene Therapy Approaches

438 views
A Detailed Protocol for Bacterial Artificial Chromosome Recombineering to Study Essential Genes in Stem Cells

436 views
Analysis of Embryonic Stem Cell-Derived Osteogenic Cultures

433 views
The Role of the Adapter Protein SHB in Embryonic Stem Cell Differentiation Into the Pancreatic β-Cell and Endothelial Lineages

430 views
Transmigration of Human CD34+ Cells

430 views
Differentiation of Human Embryonic Stem Cells Toward the Chondrogenic Lineage

430 views
Ex Vivo Expansion of Hematopoietic Stem Cells from Human Cord Blood in Serum-Free Conditions

423 views
Directed Differentiation of Human Embryonic Stem Cells to Dendritic Cells

422 views
Manipulation of Self-Renewal in Human Embryonic Stem Cells Through a Novel Pharmacological GSK-3 Inhibitor

421 views
Feeder-Layer Free Culture System for Human Embryonic Stem Cells

419 views
Derivation of Human Embryonic Stem Cells in Xeno-Free Conditions

417 views
Human Embryonic Stem Cell Differentiation on Periodontal Ligament Fibroblasts

409 views
Methodologies in Assaying Prostate Cancer Stem Cells

406 views
Differential Gene Expression in ES-Derived Neural Stem Cells by Using RT-PCR

406 views
The Expanding Tool Kit for Hematopoietic Stem Cell Research

399 views
Isolation and Characterization of Human Embryonic Stem Cells

391 views
Characterization of Nonmalignant and Malignant Prostatic Stem/Progenitor Cells by Hoechst Side Population Method

290 views
Genome-Wide DNA Methylation Profiling: The mDIP-Chip Technology

287 views
Neurosphere Culture and Human Organotypic Model to Evaluate Brain Tumor Stem Cells

272 views
Generation of Neural Crest Cells and Peripheral Sensory Neurons from Human Embryonic Stem Cells

269 views
Transplantation of Human Embryonic Stem Cells and Derivatives to the Chick Embryo

269 views
Human Embryo Culture and Assessment for the Derivation of Embryonic Stem Cells (ESC)

263 views
Methods for Analysis of Brain Tumor Stem Cell and Neural Stem Cell Self-Renewal

258 views
Culture and Preparation of Human Embryonic Stem Cells for Proteomics-Based Applications

257 views
Analysis of Cancer Stem Cell Metastasis in Xenograft Animal Models

256 views
The Contribution of Niche-Derived Factors to the Regulation of Cancer Cells

256 views
Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Embryos

253 views
Anchorage-Independent Growth of Prostate Cancer Stem Cells

252 views
Immunoflourescence and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free Conditions

249 views
Maintaining and Engineering Neural Stem Cells for Delivery of Genetically Encoded Therapy to Brain Tumors

241 views
Cancer Stem Cells in Head and Neck Squamous Cell Carcinoma

239 views
Identification of Murine and Human Acute Myeloid Leukemia Stem Cells

238 views
Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR

234 views
Vascular Differentiation of Human Embryonic Stem Cells in Bioactive Hydrogel-Based Scaffolds

233 views
Pituitary Adenoma Stem Cells

233 views
Genetic Manipulation of Human Embryonic Stem Cells

232 views
Genetic Manipulation of Human Embryonic Stem Cells in Serum and Feeder-Free Media

228 views
Serum-Free and Feeder-Free Culture Expansion of Human Embryonic Stem Cells

227 views
hESC Engineering by Integrase-Mediated Chromosomal Targeting

227 views
In Vitro Derivation of Chondrogenic Cells from Human Embryonic Stem Cells

227 views
Dendritic Cell Vaccines for Cancer Stem Cells

226 views
Identification of Human Pancreatic Cancer Stem Cells

225 views
Embryonic Stem Cells as a Model for Studying Melanocyte Development

224 views
Single Cell Enzymatic Dissociation of Human Embryonic Stem Cells: A Straightforward, Robust, and Standardized Culture Method

220 views
In Vitro Neural Differentiation of Human Embryonic Stem Cells Using a Low-Density Mouse Embryonic Fibroblast Feeder Protocol

219 views
Human-Induced Pluripotent Stem Cells: Derivation, Propagation, and Freezing in Serum- and Feeder Layer-Free Culture Conditions

216 views
Defining Cancer Stem Cells by Xenotransplantation in Zebrafish

214 views
Optimization of Physiological Xenofree Molecularly Defined Media and Matrices to Maintain Human Embryonic Stem Cell Pluripotency

213 views
Prospective Identification of Cancer Stem Cells with the Surface Antigen CD133

213 views
Growth of Human Embryonic Stem Cells Using Derivates of Human Fibroblasts

207 views
Measurement of Multiple Drug Resistance Transporter Activity in Putative Cancer Stem/Progenitor Cells

205 views
Differentiation of Neural Precursors and Dopaminergic Neurons from Human Embryonic Stem Cells

193 views
A Two- and Three-Dimensional Approach for Visualizing Human Embryonic Stem Cell Differentiation

178 views

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